Lab Methods sample questions

Question: True or False: An opportunistic pathogen can cause disease in a healthy young adult.

Answer Choices:
True
False

Answer: True

Question: Which of the following scientist(s) devised the technique to differentiate bacteria into two categories?

Answer Choices:
Watson & Crick
Robert Hooke
Antonie van Leeuwenhoek
Hans Christian Gram

Answer: Hans Christian Gram

Question: The Lac operon is an example of an [blank] operon in which the presence of lactose allows for the transcription of the beta-galactosidase gene.

Answer: inducible

Question: You have a 60X objective lens in place, and the numerical aperture of the objective lens is 0.75. The numerical aperture of the condenser lens is 1.25. What is the total magnification if the ocular lens is 10X, with the eyepieces spaced 3 cm apart?
6X

Answer Choices:
60X
600X
6000X

Answer: 600X

Question: Heat fixation is an important part of the preparation of a bacterial smear.

Answer Choices:
True
False

Answer: True

Question: __________ microscopy involves the use of dyes that must be exposed to UV light.

Answer Choices:
Electron
Bright field
Phase contrast
Fluorescent

Answer: Fluorescent

Question: A larger zone of inhibition indicates that a product is a better disinfecting/antimicrobial agent.

Answer Choices:
True
False

Answer: True

Question: You inoculated a Colilert™ tube with an unknown bacterium. After incubation, you notice that the Colilert™ medium has turned from colorless to yellow. Which of the following enzymes are responsible for this color change?

Answer Choices:
Glucuronidase
β-galactosidase
Amylase
Catalase

Answer: β-galactosidase

Question: A positive catalase test result would depict [blank] production due to release of oxygen.

Answer: bubble

Question: Use the diagram below to answer this question: The entire diagram depicts the cell envelope from a typical ________ cell.

Answer Choices:
Gram-positive bacteria
Archaea
Gram-negative bacteria
Eukaryotic
None of these are correct

Answer: Gram-negative bacteria

Question: The type of microscopy in which the field is bright and the specimen appears opaque, is known as __________-field microscopy.

Answer: Bright

Question: A membrane filter technique is used to collect bacteria from a water sample.

Answer Choices:
True
False

Answer: True

Question: The coliform-type bacteria are indicated by the blue colony color on mFC agar.

Answer Choices:
True
False

Answer: True

Question: Which quadrant in a Quadrant Streak is the least concentrated and contains isolated colonies?

Answer Choices:
Quadrant 1
Quadrant 2
Quadrant 4
Quadrant 3

Answer: Quadrant 4

Question: Horizontal transmission of symbionts occurs when the host acquires its symbionts from:
Inheritance from parent organisms

Answer Choices:
The environment
Both
Neither

Answer: The environment

Question: Mannitol salt agar (MSA) selects for organisms that can tolerate high and differentiates organisms that can ferment _.

Answer Choices:
salt; mannitol
pH; mannitol
osmotic; lactose
carbon dioxide; glucose

Answer: salt; mannitol

Question: True or False: The minimum time and temperature requirements for autoclaving are: 15 minutes at 121 °C.

Answer Choices:
True
False

Answer: True

Question: Which cellular structure is fundamentally different between Gram positive and negative bacteria and impacts the differential staining during the Gram Stain procedure?
Peptidoglycan

Answer Choices:
Outer Membrane
Chitin
Cytoplasm

Answer: Outer Membrane

Question: What is the total magnification of an image, if you are using the 100X objective lens with an ocular lens magnification power of 10X?

Answer Choices:
10X
100X
1000X
1X

Answer: 1000X

Question: The lens which is located directly ABOVE the microscope stage is called the:
Ocular

Answer Choices:
Condenser
Eyepiece
Objective

Answer: Condenser

Question: The net number of ATP generated during glycolysis is , but the total number of ATP generated during glycolysis is __ than this.

Answer Choices:
4, greater
6, less
2, less
2, greater

Answer: 2, greater

Question: True or False: The coral/Symbiodinium symbiosis is an example of vertical transfer of symbionts.

Answer Choices:
True
False

Answer: False

Question: You have a culture with a cell density of 6.7 x 10^3 CFU/mL. What final dilution factor would give you countable plates from this culture?

Answer Choices:
10^5
10^6
10^7
10^8
10^9

Answer: 10^6

Question: Amylase is an exoenzyme that breaks down starch.

Answer Choices:
True
False

Answer: True

Question: Which of the following describes the appearance of a Colilert tube that has been inoculated with E. coli?

Answer Choices:
clear liquid that fluoresces blue under UV light
clear liquid that does not fluoresce under UV light
yellow liquid that does not fluoresce under UV light
yellow liquid that fluoresces blue under UV light

Answer: yellow liquid that fluoresces blue under UV light

Lab Methods sample questions

Question: True or False: An opportunistic pathogen can cause disease in a healthy young adult.

Question: Which of the following scientist(s) devised the technique to differentiate bacteria into two categories?

Question: The Lac operon is an example of an [blank] operon in which the presence of lactose allows for the transcription of the beta-galactosidase gene.

Question: You have a 60X objective lens in place, and the numerical aperture of the objective lens is 0.75. The numerical aperture of the condenser lens is 1.25. What is the total magnification if the ocular lens is 10X, with the eyepieces spaced 3 cm apart? 6X

Question: Heat fixation is an important part of the preparation of a bacterial smear.

Question: __________ microscopy involves the use of dyes that must be exposed to UV light.

Question: A larger zone of inhibition indicates that a product is a better disinfecting/antimicrobial agent.

Question: You inoculated a Colilert™ tube with an unknown bacterium. After incubation, you notice that the Colilert™ medium has turned from colorless to yellow. Which of the following enzymes are responsible for this color change?

Question: A positive catalase test result would depict [blank] production due to release of oxygen.

Question: Use the diagram below to answer this question: The entire diagram depicts the cell envelope from a typical ________ cell.

Question: The type of microscopy in which the field is bright and the specimen appears opaque, is known as __________-field microscopy.

Question: A membrane filter technique is used to collect bacteria from a water sample.

Question: The coliform-type bacteria are indicated by the blue colony color on mFC agar.

Question: Which quadrant in a Quadrant Streak is the least concentrated and contains isolated colonies?

Question: Horizontal transmission of symbionts occurs when the host acquires its symbionts from: Inheritance from parent organisms

Question: Mannitol salt agar (MSA) selects for organisms that can tolerate high ______ and differentiates organisms that can ferment _______.

Question: True or False: The minimum time and temperature requirements for autoclaving are: 15 minutes at 121 °C.

Question: Which cellular structure is fundamentally different between Gram positive and negative bacteria and impacts the differential staining during the Gram Stain procedure? Peptidoglycan

Question: What is the total magnification of an image, if you are using the 100X objective lens with an ocular lens magnification power of 10X?

Question: The lens which is located directly ABOVE the microscope stage is called the: Ocular

Question: The net number of ATP generated during glycolysis is ________, but the total number of ATP generated during glycolysis is __________ than this.

Question: True or False: The coral/Symbiodinium symbiosis is an example of vertical transfer of symbionts.

Question: You have a culture with a cell density of 6.7 x 10^3 CFU/mL. What final dilution factor would give you countable plates from this culture?

Question: Amylase is an exoenzyme that breaks down starch.

Question: Which of the following describes the appearance of a Colilert tube that has been inoculated with E. coli?

Question: You have a 60X objective lens in place, and the numerical aperture of the objective lens is 0.75. The numerical aperture of the condenser lens is 1.25. What is the total magnification if the ocular lens is 10X, with the eyepieces spaced 3 cm apart?
6X

Question: Horizontal transmission of symbionts occurs when the host acquires its symbionts from:
Inheritance from parent organisms

Question: Mannitol salt agar (MSA) selects for organisms that can tolerate high and differentiates organisms that can ferment _.

Question: Which cellular structure is fundamentally different between Gram positive and negative bacteria and impacts the differential staining during the Gram Stain procedure?
Peptidoglycan

Question: The lens which is located directly ABOVE the microscope stage is called the:
Ocular

Question: The net number of ATP generated during glycolysis is , but the total number of ATP generated during glycolysis is __ than this.