Question: An isolation streak is not necessary for obtaining a pure culture.
Answer Choices:
True
False
Answer: False
Question: The key differentiating (most crucial) step in the Gram stain is the application of Gram’s iodine.
Answer Choices:
True
False
Answer: True
Question: Which of the following represents a chemical substance that is produced and secreted by microorganisms that inhibits or kills other microorganisms (including pathogenic microorganisms)?
Answer Choices:
Coenzyme
Antibiotic
Disinfectant
Antiseptic
Answer: Antibiotic
Question: In order to enumerate the bacteria present in a water sample, a serial dilution technique is used along with the membrane filter.
Answer Choices:
True
False
Answer: True
Question: Which of the following characteristics is NOT descriptive of the coliform group of bacteria?
Answer Choices:
Spore forming
Gram negative
Rod shaped cell
Lactose fermenting
Answer: Spore forming
Question: Uptake of naked DNA by a bacterium is:
Conjugation
Answer Choices:
Transfection
Transduction
Transformation
Answer: Transformation
Question: If the growth in an agar deep appears throughout AND more concentrated on the surface of the deep, how would you describe the aerotolerance of the bacteria?
Answer Choices:
Facultative anaerobe
Facultative aerobe
Obligate aerobe
Obligate anaerobe
Answer: Facultative anaerobe
Question: What is the total magnification when using the 40X objective lens of a microscope with an ocular magnification of 5X?
8X
Answer Choices:
200X
45X
400X
Answer: 200X
Question: The Gram stain tells us the genus of a bacterial species
Answer Choices:
True
False
Answer: False
Question: Buchnera provide their insect host with what kind of benefit?
Answer Choices:
Camouflage
Nutritional
Immune defense
Answer: Nutritional
Question: The Gram stain provides information about colony morphology.
Answer Choices:
True
False
Answer: False
Question: You are a new member of the Micro Lab prep staff. In order to get the best media making assignments for the semester, Professor Waters sets up a simple test: You must calculate the number of cells that grow in a bacterial culture over the course of an 8 hour incubation period. Here’s what you know:
The culture was started with 68 cells.
The generation time of this organism is 30 minutes.
The incubation period is 8 hours.
What is the total number of cells in the culture at the end of the 8 hour incubation period?
Answer Choices:
32,640 cells
4,462,944 cells
6,323,786,496 cells
16,320 cells
Answer: 6,323,786,496 cells
Question: When moving from a low power objective lens to a higher power objective lens, the field of view that is observed decreases.
Answer Choices:
True
False
Answer: True
Question: Coliforms are Gram-negative lactose-fermenting rods.
Answer Choices:
True
False
Answer: True
Question: After growth of the E. coli culture, ONPG was added to each of the three different carbon source tubes in the induction experiment. A positive result indicated the presence of what enzyme?
Glucoamylase
Answer Choices:
Amylase
Oxidase
β-Galactosidase
Answer: β-Galactosidase
Question: Yeast colonies resemble bacteria colonies.
Answer Choices:
True
False
Answer: True
Question: What is the anticodon of a tRNA molecule that ‘carries’ methionine (MET)?
AUG
Answer Choices:
UAC
GUA
CAU
Answer: CAU
Question: The ___ step is the most crucial and frequent source of Gram stain inconsistency.
Answer Choices:
primary stain
decolorization
heat fixation
inoculation
Answer: decolorization
Question: What is the name of the method used to test the susceptibility of bacteria to antibiotics?
Fleming Method
Answer Choices:
Henrick-Winters Method
Mueller-Hinton Method
Kirby-Bauer Method
Answer: Kirby-Bauer Method
Question: Which is the most efficient energy source glucose OR starch for Bacillus subtilis as a source of energy?
Glucose
Starch
Answer: Glucose
Question: Which of the following can develop antibiotic resistance:
Answer Choices:
Humans
Viruses
Bacteria
Fungi
All of the answers are correct
Answer: Bacteria
Question: Which is the most efficient energy source glucose OR starch for Bacillus subtilis as a source of energy?
Answer: glucose
Question: An enzyme functions by:
Answer Choices:
Creating the products of a chemical reaction.
Lowering the activation energy of a chemical reaction.
Causing a chemical reaction to occur.
Inducing gene expression.
Answer: Lowering the activation energy of a chemical reaction.
Question: Which of the following represents a chemical substance that is produced and secreted by microorganisms that inhibits or kills other microorganisms (including pathogenic microorganisms)?
Answer Choices:
Antiseptic
Disinfectant
Antibiotic
Answer: Antibiotic
Question: What is the total magnification when using the 40X objective lens of a microscope with an ocular magnification of 5X?
40X
Answer Choices:
45X
81X
200X
Answer: 200X