Answer Options:
Crystal violet
Hydrogen peroxide
Potassium hydroxide
Answer: Potassium hydroxide
Question: The bacterial genome contains ________ chromosome(s) with a ________ shape.
Answer Options:
one; linear
multiple; linear
one; circular
multiple; circular
Answer: one; circular
Question: You want to determine the cell density of a liquid bacterial culture so you perform a serial dilution. You inoculate TSA plates with 100 µl aliquots from dilution tubes. After incubation, you count the colonies on each plate and obtain the results listed below. Which plate should be chosen to calculate the original cell density (OCD)?
Answer Options:
Plate A: TNTC
Plate B: 360 colonies
Plate C: 60 colonies
Plate D: 10 colonies
Answer: Plate C: 60 colonies
Question: True or False: An opportunistic pathogen can cause disease in a healthy young adult.
Answer Options:
True
False
Answer: True
Question: Approximately how many CFU would you expect to count if you plated 1.0 mL of a broth with a cell density of 3.52 x 10^2 CFU/mL?
Answer Options:
35 CFU
3.52 CFU
352 CFU
0.352 CFU
Answer: 352 CFU
Question: The function of the “ciliary escalator” is to:
Answer: propel inhaled dust and microorganisms toward the mouth, away from the lower respiratory tract.
Question: A broth has 2.20 x 10^8 CFU/mL. Approximately how many CFUs would you expect to count if you plated 0.5 mL? Please provide WHOLE number, NO EXPONENTS.
Answer Options:
220 CFU
22 CFU
2 CFU
110 CFU
Answer: 110000000
Question: True or False: The glycocalyx is the outermost boundary for both eukaryotic and prokaryotic microbial cells.
Answer Options:
True
False
Answer: True
Question: True or False: The minimum time and temperature requirements for autoclaving are: 15 minutes at 121 °C.
Answer Options:
True
False
Answer: True
Question: What happens in diabetes mellitus?
Answer Options:
A defect in insulin’s action.
Both a and b are correct.
A deficit in insulin secretion.
Answer: Both a and b are correct.
Question: Endo agar is both selective and differential. The agar selects for ______ bacteria and differentiates those that grow by their ability to ______.
Answer Options:
Gram-positive, ferment lactose
Acid fast, produce endospores
Anaerobic, reduce nitrate
Gram-negative, ferment lactose
Answer: Gram-negative, ferment lactose
Question: Which antimicrobial target would result in high (good) selective toxicity for that drug?
Answer Options:
Cell membrane function
Peptidoglycan synthesis
Nucleic acid synthesis
None of these
Answer: Peptidoglycan synthesis
Question: True or False: All proteins have tertiary structure.
Answer Options:
True
False
Answer: True
Question: Amylase is an exoenzyme that breaks down starch. True False
Answer: True