Question: Which of the following can develop antibiotic resistance:

Answer Options:
Humans
Viruses
Bacteria
Fungi
All of the answers are correct

Answer: Bacteria

Question: Using the Kirby-Bauer antibiotic sensitivity test, a single plate can be used to determine

Answer Options:
the sensitivity of a particular bacterium to a number of different antibiotics
the sensitivity of many strains of bacteria to a number of different antibiotics
all of these
the sensitivity of many strains of bacteria to a single antibiotic

Answer: all of these

Question: The three domains of life are the Bacteria, Eukarya, and the:

Answer Options:
Monera
Protista
Fungi
Viruses
Archaea

Answer: Archaea

Question: The acquisition of genetic material through the uptake of “naked” DNA molecules from the environment by bacteria is called:

Answer Options:
conjugation
transformation
regeneration
transduction

Answer: transformation

Question: What is the purpose of using a decolorizer in the gram stain procedure?

Answer Options:
It removes crystal violet from gram positive cells.
It removes crystal violet from gram negative cells.
It removes safranin from gram positive cells.
It makes the primary stain adhere more strongly.
It fixes bacteria on the slide.

Answer: It removes crystal violet from gram negative cells.

Question: You inoculated an agar deep with an unknown culture and observed growth ONLY in the bottom of the tube after incubation. How would you describe the aerotolerance of this microorganism?

Answer Options:
Facultative anaerobe
Obligate anaerobe
Obligate aerobe

Answer: Obligate anaerobe

Question: “Microbes are everywhere” is another way of saying that microbes are ____________.

Answer Options:
inoculating
aseptic
exposed
ubiquitous
undulating

Answer: ubiquitous

Question: Which of the following types of cultures should be used for subculture (transfer) to a new medium to obtain a pure culture?

Answer Options:
a loopful of broth from a broth culture
a swab of growth taken from quadrant 1 of a quadrant streak plate
More than one of these is correct
0.1 ml of broth from a broth culture
an isolated colony on an agar streak plate or pour plate

Answer: an isolated colony on an agar streak plate or pour plate

Question: How many bacterial cells are necessary and sufficient to establish one colony on an agar plate?

Answer Options:
Between 30 and 300
few
at least 10
one

Answer: one

Question: The pathogen Staphylococcus aureus is only found on people’s skin if they have an infection.

Answer Options:
True
False

Answer: False