Question: You have a culture with a cell density of 6.7 x 10^3 CFU/mL. What final dilution factor would give you countable plates from this culture?

Answer Options:
10^5
10^6
10^7
10^8
10^9

Answer: 10^6

Question: A positive catalase test result would depict [blank] production due to release of oxygen.

Answer: bubble

Question: The following are Koch’s Postulates, in random order. Which is the correct order?

(1) The suspected organism is grown in pure culture
(2) Cells from a pure culture of the suspected organism should cause disease in a healthy animal
(3) The organism is re-isolated and shown to be the same as the original
(4) The suspected pathogen should be present in all cases of the disease and absent from healthy animals.

1, 3, 2, 4
3, 4, 1, 2
3, 1, 2, 4
4, 1, 2, 3
1, 2, 4, 3

Answer: 4, 1, 2, 3

Question: Which of the following is the most exclusive hierarchical taxonomic group?

Answer Options:
Species
Family
Genus
Phylum

Answer: Species

Question: One mL of a sample had been diluted to 10^-8 and was spread on a TSA plate. The plate yielded 80 CFU. What is the original cell density (OCD)?

Answer Options:
80 x 10^8 CFU/mL
8 x 10^8 CFU/mL

Answer Options:
80 x 10^8 CFU/mL
8 x 10^8 CFU/mL

Answer: 80 x 10^8 CFU/mL

Question: What are the mutants/mL of a TSA + streptomycin agar plate that contains 1.5 mL of sample and 25 mutant colonies (rounded to nearest whole)?

Answer Options:
38 mutants/mL
17 mutants/mL

Answer Options:
38 mutants/mL
17 mutants/mL

Answer: 17 mutants/mL

Question: Approximately how many CFU would you expect to count if you plated 1.0 mL of a broth with a cell density of 3.52 x 10^2 CFU/mL?

Answer Options:
35 CFU
3.52 CFU
352 CFU
0.352 CFU

Answer: 352 CFU

Question: Wernicke encephalopathy/thiamine (Vit B1) deficiency is a side effect of which substance use?

Meth
Cocaine
Alcohol
Marijuana

Answer: Alcohol

Question: A segment of DNA that codes for the synthesis of an RNA molecule that will then code for a specific protein is a ________.

Answer Options:
lysosome
plasmid
gene
chromosome

Answer: gene

Question: Atherosclerosis/arteriosclerosis can be a cause of vascular dementias
True
False

Answer: True

Question: Which medicine/drug is used to counteract the side-effects of overdosing opioid drugs?

Naloxone
Penicillin
Nicotine
Ethyl alcohol

Answer: Naloxone

Question: Calculate the total magnification if you are using a microscope with an ocular magnification of 5x and an objective magnification of 200x.

Answer Options:
250x
5x
200x
1000x

Answer: 1000x

Question: You want to determine the cell density of a liquid bacterial culture so you perform a serial dilution. You inoculate TSA plates with 100 µl aliquots from dilution tubes. After incubation, you count the colonies on each plate and obtain the results listed below. Which plate should be chosen to calculate the original cell density (OCD)?

Answer Options:
Plate A: TNTC
Plate B: 360 colonies
Plate C: 60 colonies
Plate D: 10 colonies

Answer: Plate C: 60 colonies

Question: After incubation of an inoculated Phenol Red Fermentation Broth tube, the test organism was characterized a glucose fermenter since the broth changed from red to [blank].

Answer: yellow

Question: Approximately how many CFU would you expect to count if you plated 0.1 mL of a broth with a cell density of 3.50 x 10^2 CFU/mL?

Answer Options:
35 CFU
3.50 CFU
350 CFU
0.35 CFU

Answer: 35 CFU

Question: A countable plate contains _____ to _____ colonies (numerical format).

Answer: 30 to 300

Question: If you flood starch agar plates with Gram’s iodine the agar will turn purple. If the starch has been broken down, a ______ will appear around the colony.

Answer Options:
Clear zone
Zone of inhibition
Yellow halo
Metallic sheen

Answer Options:
Clear zone
Zone of inhibition
Yellow halo
Metallic sheen

Answer: Clear zone

Question: Which one is the most common bipolar disorder?

Cyclothymia
Bipolar I disorder
Bipolar II disorder
Bipolar N disorder

Answer: Bipolar I disorder

Question: Which pertains to RNA but not to DNA:

Answer Options:
Contains ribose
Contains adenine
Contains thymine
Contains guanine

Answer: Contains ribose

Question: Which of the following is measured to determine the zone of inhibition?

Answer Options:
Volume
Diameter
Radius
Area

Answer Options:
Volume
Diameter
Radius
Area

Answer: Diameter

Question: The symbiotic relationship between Aliivibrio fischeri and the Hawaiian Bobtail squid is:

Answer Options:
Commensal
Mutualistic
Amensal
Predatory

Answer: Mutualistic

Question: Which quadrant in a Quadrant Streak is the least concentrated and contains isolated colonies?

Answer Options:
Quadrant 1
Quadrant 2
Quadrant 4
Quadrant 3

Answer: Quadrant 4

Question: Which one is the most frequently occurring psychiatric condition?

Depressive disorder
Anxiety disorder
Paranoia
Mania

Answer: Anxiety disorder

Question: Plant-like protist are termed [blank], while animal-like protist are termed [blank].

Answer: protohyta
protozoa